HPLC determination of photosynthetic pigments during greening of etiolated barley leaves. Evidence for the biosynthesis of chlorophyll a'.


The temporal evolution of pigment composition during greening of etiolated barley leaves was investigated by reversed-phase HPLC with particular attention to chlorophyll (Chl) a' (C13(2) epimer of Chl a), which had been detected by ourselves in photosystem (PS) I particles. At early stages of greening, the Chl a'/Chl a molar ratio rapidly increased to a… (More)


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