HPLC-based kinetics assay facilitates analysis of systems with multiple reaction products and thermal enzyme denaturation.

@article{Klingaman2017HPLCbasedKA,
  title={HPLC-based kinetics assay facilitates analysis of systems with multiple reaction products and thermal enzyme denaturation.},
  author={Chase A. Klingaman and Matthew J. Wagner and Justin R. Brown and John B. Klecker and Ethan H. Pauley and Colin J. Noldner and Jared R Mays},
  journal={Analytical biochemistry},
  year={2017},
  volume={516},
  pages={37-47}
}
Glucosinolates are plant secondary metabolites abundant in Brassica vegetables that are substrates for the enzyme myrosinase, a thioglucoside hydrolase. Enzyme-mediated hydrolysis of glucosinolates forms several organic products, including isothiocyanates (ITCs) that have been explored for their beneficial effects in humans. Myrosinase has been shown to be tolerant of non-natural glucosinolates, such as 2,2-diphenylethyl glucosinolate, and can facilitate their conversion to non-natural ITCs… CONTINUE READING