HPLC-based activity profiling of Angelica pubescens roots for new positive GABAA receptor modulators in Xenopus oocytes.

@article{Zaugg2011HPLCbasedAP,
  title={HPLC-based activity profiling of Angelica pubescens roots for new positive GABAA receptor modulators in Xenopus oocytes.},
  author={Janine Zaugg and Eva Eickmeier and Diana Rueda and Steffen Hering and Matthias Hamburger},
  journal={Fitoterapia},
  year={2011},
  volume={82 3},
  pages={434-40}
}
A petroleum ether extract of the traditional Chinese herbal drug Duhuo (roots of Angelica pubescens Maxim. f. biserrata Shan et Yuan), showed significant activity in a functional two-microelectrode voltage clamp assay with Xenopus oocytes which expressed recombinant γ-aminobutyric acid type A (GABA(A)) receptors of the subtype α(1)β(2)γ(2S). HPLC-based activity profiling of the active extract revealed six compounds responsible for the GABA(A) receptor modulating activity. They were identified… CONTINUE READING