HPLC analysis of putative amino acid neurotransmitters released from primary cerebellar cultures

@article{Rogers1987HPLCAO,
  title={HPLC analysis of putative amino acid neurotransmitters released from primary cerebellar cultures},
  author={Keith Leslie Rogers and Robert A Philibert and Albert J. Allen and Jerry A. Molitor and Gary R. Dutton},
  journal={Journal of Neuroscience Methods},
  year={1987},
  volume={22},
  pages={173-179}
}
An HPLC method is described that measures amino acids (putative neurotransmitters and/or neuromodulators) released from primary, dissociated cerebellar cells maintained in monolayer culture. Precolumn derivatization with phenylisothiocyanate, followed by reverse phase chromatography with UV detection was used to quantitate the phenylcarbamyl amino acid derivatives in a chemically defined medium. Quantitation was linear, reproducible and sensitive to one picomole. This method is useful for the… CONTINUE READING
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