HPLC-F analysis of melatonin and resveratrol isomers in wine using an SPE procedure.

@article{Mercolini2008HPLCFAO,
  title={HPLC-F analysis of melatonin and resveratrol isomers in wine using an SPE procedure.},
  author={Laura Mercolini and Maria Addolorata Saracino and Francesca Bugamelli and Anna Ferranti and Marco Malaguti and Silvana Hrelia and Maria Augusta Raggi},
  journal={Journal of separation science},
  year={2008},
  volume={31 6-7},
  pages={1007-14}
}
An original analytical method has been developed for the determination of the antioxidants trans-resveratrol (t-RSV) and cis-resveratrol (c-RSV) and of melatonin (MLT) in red and white wine. The method is based on HPLC coupled to fluorescence detection. Separation was obtained by using a RP column (C8, 150 mm x 4.6 mm id, 5 mum) and a mobile phase composed of 79% aqueous phosphate buffer at pH 3.0 and 21% ACN. Fluorescence intensity was monitored at lambda = 386 nm while exciting at lambda… CONTINUE READING
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