HPLC Determination of Esculin and Esculetin in Rat Plasma for Pharmacokinetic Studies.

@article{Rehman2015HPLCDO,
  title={HPLC Determination of Esculin and Esculetin in Rat Plasma for Pharmacokinetic Studies.},
  author={Shaheed Ur Rehman and In Sook Kim and Ki Sung Kang and Hye Hyun Yoo},
  journal={Journal of chromatographic science},
  year={2015},
  volume={53 8},
  pages={
          1322-7
        }
}
  • S. Rehman, I. Kim, +1 author H. Yoo
  • Published 1 September 2015
  • Chemistry, Medicine
  • Journal of chromatographic science
An optimized, sensitive and validated reversed-phase high-performance liquid chromatography (RP-HPLC) method with UV detection is described for simultaneous determination of esculin and its aglycone, esculetin, in rat plasma. After addition of internal standard (chrysin), plasma samples were pretreated by solid-phase extraction and introduced into the HPLC system. Analytes were separated on a RP C18 column with a mobile phase of 0.075% acetic acid in water (solvent A) and 90% acetonitrile in… 
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Determination of aesculin in rat plasma by high performance liquid chromatography method and its application to pharmacokinetics studies.
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TLDR
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TLDR
Aesculin can be transferred into aesculetin by human gut bacteria and is further modified by the host in vivo, which may explain its pleiotropic pharmaceutical effects.
Inhibitory effect of natural coumarin compounds, esculetin and esculin, on oxidative DNA damage and formation of aberrant crypt foci and tumors induced by 1,2-dimethylhydrazine in rat colons.
TLDR
It is suggested that esculin intake has an inhibitory effect on DMH-induced oxidative DNA damage and carcinogenesis in rat colons, and the ingestion of escul in the initiation phase significantly reduced the incidence of gross tumors, the number ofACF per rat and the mean number of AC per focus.
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TLDR
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