HPLC Determination and Pharmacokinetic Study of Valdecoxib in Human Plasma

@article{Sane2005HPLCDA,
  title={HPLC Determination and Pharmacokinetic Study of Valdecoxib in Human Plasma},
  author={Ramesh T. Sane and Sasikumar N. Menon and Abhijeet Yashwantrao Deshpande and A. Jain},
  journal={Chromatographia},
  year={2005},
  volume={61},
  pages={137-141}
}
A sensitive, simple, and accurate high-performance liquid chromatographic method has been developed for determination of valdecoxib and the internal standard rofecoxib in human plasma. Protein was precipitated from plasma samples by addition of perchloric acid (HClO4); the drug was then extracted with diethyl ether. Separation was performed on a Cosmosil C18 column (150 mm × 4.6 mm i.d., 5 μm particles) with ammonium acetate buffer-acetonitrile, 60:40 (v/v), containing 0.1% TEA, pH 6.5, as… 
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The data presented here is the first LC-MS/MS method for the quantitation of firocoxib in plasma (LLOQ of 1 ng/mL), a 25-fold improvement in sensitivity over the HPLC-UV method and the first quantitative method for firo Coxib in urine (LLoQ of 5 ng/ mL).
Validation of an HPLC Method for the Determination of Valdecoxib and its Degradation Product: a Mixture of α- and β-n-Lactosyl Sulfonamide Anomers
An HPLC method has been developed for the separation of valdecoxib and a degradation product consisting of α and β-N-lactosyl sulfonamide, i.e. α and β anomers (SC-77852). Best results were achieved
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References

SHOWING 1-8 OF 8 REFERENCES
Determination of candesartan cilexetil, candesartan and a metabolite in human plasma and urine by liquid chromatography and fluorometric detection.
Liquid chromatographic methods are described for the determination of a new effective anti-hypertensive drug candesartan (CV-11974), its prodrug candesartan cilexetil (TCV-116) and a metabolite,
Development and validation of an automated SPE-LC-MS/MS assay for valdecoxib and its hydroxylated metabolite in human plasma.
TLDR
A sensitive and specific liquid chromatography-tandem mass spectrometry assay was developed to quantitate valdecoxib and its hydroxylated metabolite and has been successfully used to analyze human plasma samples to support clinical phase I and II studies.
Characterization of conjugated metabolites of a new angiotensin II receptor antagonist, candesartan cilexetil, in rats by liquid chromatography/electrospray tandem mass spectrometry following chemical derivatization.
TLDR
The results obtained in this study indicated that LC/ESI-MS/MS analysis provides the detailed structure of conjugated metabolite by simple chemical derivatization.
Practical HPLC method development
Getting Started. Basics of Separation. Detection Sensitivity and Selectivity. Sample Preparation. The Column. Non--Ionic Samples: Reversed-- and Normal--Phase HPLC. Ionic Samples: Reversed--Phase,
Design and Analysis of Bioavailability and Bioequivalence Studies
Preface Preliminaries Introduction History of Bioavailability Studies Formulation and Routes of Administration Pharmacokinetic Parameters Clinically Important Differences Assessment of Bioequivalence
Chemical analysis in complex matrices
Chemical analysis in complex matrices - an introduction drug analysis in biological fluids analysis in the brewing industry the analytical laboratory in the speciality sealants/adhesives industry air