HPLC Determination and Pharmacokinetic Study of Valdecoxib in Human Plasma

  title={HPLC Determination and Pharmacokinetic Study of Valdecoxib in Human Plasma},
  author={Ramesh T. Sane and Sasikumar N. Menon and Abhijeet Yashwantrao Deshpande and A. Jain},
A sensitive, simple, and accurate high-performance liquid chromatographic method has been developed for determination of valdecoxib and the internal standard rofecoxib in human plasma. Protein was precipitated from plasma samples by addition of perchloric acid (HClO4); the drug was then extracted with diethyl ether. Separation was performed on a Cosmosil C18 column (150 mm × 4.6 mm i.d., 5 μm particles) with ammonium acetate buffer-acetonitrile, 60:40 (v/v), containing 0.1% TEA, pH 6.5, as… 
Quantitative HPLC-UV method for the determination of firocoxib from horse and dog plasma.
A sensitive reversed-phase HPLC-UV method was developed for the determination of firocoxib, a novel and highly selective COX-2 inhibitor, in plasma and is suitable for the analysis of clinical samples from pharmacokinetic and bioequivalence studies and drug monitoring.
Simultaneous estimation of cefixime and cloxacillin in human plasma by reversed phase-HPLC with UV detection
An accurate, rapid and simple reversed-phase high performance liquid chromatography (RP-HPLC) method was developed and validated for simultaneous estimation of cefixime, cloxacillin and for
Simultaneous detection and quantification of parecoxib and valdecoxib in canine plasma by HPLC with spectrofluorimetric detection: development and validation of a new methodology
This is the first time that use of a HPLC with spectrofluorimetric detection technique to simultaneously detect parecoxib and valdecoxib in plasma has been reported and may have applications for pharmacokinetic studies.
Validated RP-HPLC method for quantification of felodipine in rabbit plasma: Application in a bioequivalence study.
The validated RP-HPLC method was successfully applied for the bioavailability studies of felodipine and proved its efficiency to be applied to the therapeutic drug monitoring (TDM) and bioequivalence (BE) studies.
A Validated and Stability-Indicating LC Assay Method for Valdecoxib
A gradient reversed-phase liquid chromatographic assay was developed for the quantitative determination of the non-steroidal anti-inflammatory drug valdecoxib. The developed method was also
Simultaneous determination of parecoxib sodium and its active metabolite valdecoxib in rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study after intravenous and intramuscular administration.
  • M. Liu, Qiuyang Yu, +10 authors Xiaohong Liu
  • Chemistry, Medicine
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
  • 2016
A new, rapid, specific and sensitive ultra-performance liquid chromatography-tandem mass spectrometric method to simultaneously determine parecoxib sodium (PX) and its active metabolite, valdecoxib (VX), in rat plasma was developed and validated.
Meloxicam quantification in rabbit plasma by RP-HPLC: optimization and application to pharmacokinetic study
The goal of the proposed study was to validate a rapid, simple, an accurate, robust, and sensitive bioanalytical method for quantifying Meloxicam and Lornoxicam (as internal standard) in rabbit
Development and Validation of Liquid Chromatography Method for the Separation of Valdecoxib and its SC‐77852 Impurity
Abstract A reversed‐phase liquid chromatography method has been developed for the separation of valdecoxib and impurity SC‐77852. The best results were achieved using a mobile phase—methanol: 1%
Automated liquid chromatography-tandem mass spectrometry method for the analysis of firocoxib in urine and plasma from horse and dog.
The data presented here is the first LC-MS/MS method for the quantitation of firocoxib in plasma (LLOQ of 1 ng/mL), a 25-fold improvement in sensitivity over the HPLC-UV method and the first quantitative method for firo Coxib in urine (LLoQ of 5 ng/ mL).
Validation of an HPLC Method for the Determination of Valdecoxib and its Degradation Product: a Mixture of α- and β-n-Lactosyl Sulfonamide Anomers
An HPLC method has been developed for the separation of valdecoxib and a degradation product consisting of α and β-N-lactosyl sulfonamide, i.e. α and β anomers (SC-77852). Best results were achieved


Determination of candesartan cilexetil, candesartan and a metabolite in human plasma and urine by liquid chromatography and fluorometric detection.
Liquid chromatographic methods are described for the determination of a new effective anti-hypertensive drug candesartan (CV-11974), its prodrug candesartan cilexetil (TCV-116) and a metabolite,
Development and validation of an automated SPE-LC-MS/MS assay for valdecoxib and its hydroxylated metabolite in human plasma.
A sensitive and specific liquid chromatography-tandem mass spectrometry assay was developed to quantitate valdecoxib and its hydroxylated metabolite and has been successfully used to analyze human plasma samples to support clinical phase I and II studies.
Characterization of conjugated metabolites of a new angiotensin II receptor antagonist, candesartan cilexetil, in rats by liquid chromatography/electrospray tandem mass spectrometry following chemical derivatization.
The results obtained in this study indicated that LC/ESI-MS/MS analysis provides the detailed structure of conjugated metabolite by simple chemical derivatization.
Practical HPLC method development
Getting Started. Basics of Separation. Detection Sensitivity and Selectivity. Sample Preparation. The Column. Non--Ionic Samples: Reversed-- and Normal--Phase HPLC. Ionic Samples: Reversed--Phase,
Design and Analysis of Bioavailability and Bioequivalence Studies
Preface Preliminaries Introduction History of Bioavailability Studies Formulation and Routes of Administration Pharmacokinetic Parameters Clinically Important Differences Assessment of Bioequivalence
Chemical analysis in complex matrices
Chemical analysis in complex matrices - an introduction drug analysis in biological fluids analysis in the brewing industry the analytical laboratory in the speciality sealants/adhesives industry air