HPLC-DAD-MS(n) analysis and HPLC quantitation of chemical constituents in Xian-ling-gu-bao capsules.

@article{Guan2011HPLCDADMSnAA,
  title={HPLC-DAD-MS(n) analysis and HPLC quantitation of chemical constituents in Xian-ling-gu-bao capsules.},
  author={Xiang-yu Guan and Hui-fang Li and Wen-zhi Yang and Chang-hu Lin and Chao Sun and Bao-rong Wang and De-an Guo and Min Ye},
  journal={Journal of pharmaceutical and biomedical analysis},
  year={2011},
  volume={55 5},
  pages={
          923-33
        }
}
In this study, a systematic method was established for the global quality control of Xian-ling-gu-bao capsules (XLGB), a popular six-herb Traditional Chinese Patent Medicine (TCPM) for the treatment of osteoporosis. Both qualitative and quantitative analyses were conducted. In qualitative analysis part, a fast and sensitive method based on high-performance liquid chromatography coupled with diode-array detection and electrospray ionization tandem mass spectrometry (HPLC-DAD-MS(n)) was… Expand
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  • Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
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References

SHOWING 1-10 OF 22 REFERENCES
A rapid and sensitive liquid chromatography-tandem mass spectrometric method for the determination of timosaponin B-II in blood plasma and a study of the pharmacokinetics of saponin in the rat.
A rapid, sensitive and selective liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the determination of Timosaponin B-II (TB-II), a pharmacologicallyExpand
Fingerprint analysis of Psoralea corylifolia L. by HPLC and LC-MS.
TLDR
The HPLC fingerprint analysis is specific and may serve for quality identification and comprehensive evaluation of P. corylifolia and assess the differences among the herb grown in various areas of China. Expand
HPLC simultaneous determination of epimedin B,epimedin C and icariin in Xianlinggubao capsules
Objective:To develop an HPLC method for simultaneous determination of epimedin B,epimedin C and icariin in Xianlinggubao capsules.Methods:The samples were separated on Elite SinoChrom ODS-AP (250Expand
Simultaneous determination of 15 flavonoids in Epimedium using pressurized liquid extraction and high-performance liquid chromatography.
TLDR
A reliable pressurized liquid extraction and HPLC method was developed for simultaneous determination of 15 flavonoids in different species of Epimedium which had great variation on the contents of investigated flavonoid contents. Expand
Analysis of phenolic compounds in Epimedium plants using liquid chromatography coupled with electrospray ionization mass spectrometry.
TLDR
The differences in phenolic compound profiles between different Epimedium plants and two parts of the seven species were reported for the first time and should be used separately in clinical practice. Expand
Characterization of the interglycosidic linkage in di-, tri-, tetra- and pentaglycosylated flavonoids and differentiation of positional isomers by liquid chromatography/electrospray ionization tandem mass spectrometry.
TLDR
In tetra- and pentaglycosylated flavonoids, the ions that characterize the (1-->2) interglucosidic linkage formed by intermediate fragmentation of the oligosacharide residues (sophorosides and sophorotriosides) are found in much higher relative abundance in MS3 than in MS2 experiments, where they are almost not detected. Expand
STUDIES ON THE FUROSTANOL SAPONINS FROM ANEMARRHENA ASPHODELOIDES BUNGE
Four furostanol saponins were isolated brom. the ethanol extract of Anemarrhenaasphodeloides Bunge(Liliaccae),by repeated column chromatography on silica gel and preparativeHPLC. On the basis ofExpand
Application of mass spectrometry for identification and structural studies of flavonoid glycosides.
TLDR
A review of mass spectrometric techniques applied to the identification and structural studies of flavonoid glycosides is presented. Expand
New tetrasaccharide flavonol glycoside from Epimedium acuminatum.
A new tetrasaccharide flavonol glycoside was isolated from the aerial parts of Epimedium acuminatum, along with three known flavonoids. The structure of the new compound, named acuminatoside [1], wasExpand
Prenylflavonol, acylated flavonol glycosides and related compounds from Epimedium sagittatum.
TLDR
The results indicated that the n-BuOH extract of E. sagittatum produced a partial endothelial nitric oxide-dependent vasorelaxation, with EC(50) of 0.16+/-0.03 mg/ml, however, the 13 compounds tested, generated only a mild or moderate relaxation, and did not possess significant vasore laxing effect individually. Expand
...
1
2
3
...