HPLC/UV quantitation of retinal, retinol, and retinyl esters in serum and tissues.

@article{Kane2008HPLCUVQO,
  title={HPLC/UV quantitation of retinal, retinol, and retinyl esters in serum and tissues.},
  author={Maureen A. Kane and Alexandra E. Folias and Joseph L Napoli},
  journal={Analytical biochemistry},
  year={2008},
  volume={378 1},
  pages={
          71-9
        }
}
We report robust HPLC/UV methods for quantifying retinyl esters (RE), retinol (ROL), and retinal (RAL) applicable to diverse biological samples with lower limits of detection of 0.7, 0.2, and 0.2 pmol, respectively, and linear ranges greater than 3 orders of magnitude. These assays function well with small, complex biological samples (10-20mg tissue). Coefficients of variation range from 5.9 to 10.0% (intraday) and from 5.9 to 11.0% (interday). Quantification of endogenous RE, ROL, and RAL in… CONTINUE READING

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