HNF1 alpha activates the aminopeptidase N promoter in intestinal (Caco-2) cells.

@article{Olsen1994HNF1AA,
  title={HNF1 alpha activates the aminopeptidase N promoter in intestinal (Caco-2) cells.},
  author={J\orgen Olsen and Line Skall Laustsen and Jesper Thorvald Troelsen},
  journal={FEBS letters},
  year={1994},
  volume={342 3},
  pages={325-8}
}
The importance of HNF1 binding proteins for intestinal aminopeptidase N expression was investigated using the Caco-2 cell-line. Aminopeptidase N promoter activity in Caco-2 cells depends on the HNF1 element (positions -85 to -58) and co-transfection with an HNF1 alpha expression vector demonstrates a direct activation of the promoter by HNF1 alpha through this element. Electrophoretic mobility shift assays using nuclear extracts from Caco-2 cells show the presence of high amounts of HNF1… CONTINUE READING