HLA-A2 subtypes are functionally distinct in peptide binding and presentation

  title={HLA-A2 subtypes are functionally distinct in peptide binding and presentation},
  author={Dan Barouch and Thomas Friede and Stefan Stevanovi{\'c} and Lynda Tussey and Katy Smith and Sarah Rowland-Jones and V{\'e}ronique Braud and A. J. McMichael and Hans Georg Rammensee},
  journal={The Journal of Experimental Medicine},
  pages={1847 - 1856}
Nearly half of HLA-A2-positive individuals in African populations have a subtype of HLA-A2 other than the A*0201 allele. We have isolated the common African HLA-A2 subtype genes from Epstein-Barr virus-transformed B cell lines and have established stable class I reduced transfectants expressing these alleles. We have studied the peptide binding and presentation properties of A*0201, A*0202, A*0205, A*0214, and A*6901 by a combination of approaches: assaying direct binding of labeled synthetic… CONTINUE READING