HIV nucleocapsid protein. Expression in Escherichia coli, purification, and characterization.

@article{You1993HIVNP,
  title={HIV nucleocapsid protein. Expression in Escherichia coli, purification, and characterization.},
  author={Ji Chang You and Charles S McHenry},
  journal={The Journal of biological chemistry},
  year={1993},
  volume={268 22},
  pages={16519-27}
}
The single-stranded nucleocapsid protein that coats the RNA genome of human immunodeficiency virus within the virion core has been produced in Escherichia coli and purified to homogeneity. The mature 55-amino acid protein, normally generated from the gag polyprotein precursor by HIV protease-catalyzed processing of both its amino and carboxyl termini, was produced in E. coli with authentic termini directly, without the need for processing. The protein was purified 30-fold to apparent… CONTINUE READING