HIV induces lymphocyte apoptosis by a p53-initiated, mitochondrial-mediated mechanism.

@article{Genini2001HIVIL,
  title={HIV induces lymphocyte apoptosis by a p53-initiated, mitochondrial-mediated mechanism.},
  author={Davide Genini and Dennis A Sheeter and Steffney Rought and John James Zaunders and Santos A Susin and Guido Kroemer and Douglas D. Richman and Dennis Anthony Carson and Jacques Corbeil and Lorenzo M. Leoni},
  journal={FASEB journal : official publication of the Federation of American Societies for Experimental Biology},
  year={2001},
  volume={15 1},
  pages={5-6}
}
HIV-1 induces apoptosis and leads to CD4+ T-lymphocyte depletion in humans. It is still unclear whether HIV-1 kills infected cells directly or indirectly. To elucidate the mechanisms of HIV-1-induced apoptosis, we infected human CD4+ T cells with HIV-1. Enzymatic analysis with fluorometric substrates showed that caspase 2, 3, and 9 were activated in CD4+ T cells with peak levels 48 h after infection. Immunoblotting analysis confirmed the cleavage of pro-caspase 3 and 9, and of specific caspase… CONTINUE READING