• Biology, Medicine, Computer Science
  • Published in Nucleic Acids Research 2011
  • DOI:10.1093/nar/gkq794

HIV-1 RT-dependent DNAzyme expression inhibits HIV-1 replication without the emergence of escape viruses

@inproceedings{Sugiyama2011HIV1RD,
  title={HIV-1 RT-dependent DNAzyme expression inhibits HIV-1 replication without the emergence of escape viruses},
  author={Ryuichi Sugiyama and Masaaki Hayafune and Yuichiro Habu and Norio Yamamoto and Hiroshi Takaku},
  booktitle={Nucleic Acids Research},
  year={2011}
}
DNAzymes are easier to prepare and less sensitive to chemical and enzymatic degradation than ribozymes; however, a DNA enzyme expression system has not yet been developed. In this study, we exploited the mechanism of HIV-1 reverse transcription (RT) in a DNA enzyme expression system. We constructed HIV-1 RT-dependent lentiviral DNAzyme expression vectors including the HIV-1 primer binding site, the DNA enzyme, and either a native tRNA (Lys-3), tR(M)DtR(L), or one of two truncated tRNAs (Lys-3… CONTINUE READING

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