HIGH-YIELD PREPARATION OF ISOLATED RAT LIVER PARENCHYMAL CELLS

@article{Berry1969HIGHYIELDPO,
  title={HIGH-YIELD PREPARATION OF ISOLATED RAT LIVER PARENCHYMAL CELLS },
  author={Michael Nathaniel Prof. Berry and Daniel Friend},
  journal={The Journal of Cell Biology},
  year={1969},
  volume={43},
  pages={506 - 520}
}
A new technique employing continuous recirculating perfusion of the rat liver in situ, shaking of the liver in buffer in vitro, and filtration of the tissue through nylon mesh, results in the conversion of about 50% of the liver into intact, isolated parenchymal cells. The perfusion media consist of: (a) calcium-free Hanks' solution containing 0.05% collagenase and 0.10% hyaluronidase, and (b) magnesium and calcium-free Hanks' solution containing 2 mM ethylenediaminetetraacetate. Biochemical… CONTINUE READING

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