HER-2 gene amplification by fluorescence in situ hybridization (FISH) compared with immunohistochemistry (IHC) in breast cancer: a study of 528 equivocal cases

@article{Zhang2012HER2GA,
  title={HER-2 gene amplification by fluorescence in situ hybridization (FISH) compared with immunohistochemistry (IHC) in breast cancer: a study of 528 equivocal cases},
  author={Han Zhang and Guo-ping Ren and Xiaoling Wang and Jing Zhao and Hongtian Yao and Yanfeng Bai and Wang Bo},
  journal={Breast Cancer Research and Treatment},
  year={2012},
  volume={134},
  pages={743-749}
}
The concordance rate between fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) on evaluating HER-2/neu status is still controversial, especially for the IHC 2+/equivocal cases. In this study, we investigated the amplification of HER-2/neu gene by FISH in IHC (2+)/equivocal specimens to clarify the correlation between HER-2/neu and clinicopathologic features of breast cancers. HER-2/neu amplification was determined by FISH on 528 specimens of breast carcinomas with… CONTINUE READING