HEL-Flu: an influenza virus containing the hen egg lysozyme epitope recognized by CD4+ T cells from mice transgenic for an alphabeta TCR.

@article{Walker1997HELFluAI,
  title={HEL-Flu: an influenza virus containing the hen egg lysozyme epitope recognized by CD4+ T cells from mice transgenic for an alphabeta TCR.},
  author={William K. S. Walker and Maria Rita Castrucci and Mark Y. Sangster and Richard T. Carson and Yoshihiro Kawaoka},
  journal={Journal of immunology},
  year={1997},
  volume={159 6},
  pages={
          2563-6
        }
}
Reverse genetics was used to modify the influenza virus genome by inserting the p46-63 sequence of hen egg lysozyme (HEL) into the neuraminidase stalk of the virus. The resulting virus, HEL-Flu, contained the epitopes recognized by CD4+ T cells from 3A9-TCR transgenic mice (C3HTg). Here, we show that HEL-Flu was infectious in the respiratory tract of both C3H and C3HTg mice, the latter animals showing an early, transient morbidity. Splenic dendritic cells and certain cloned populations of… CONTINUE READING

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