HEAVY‐METAL INDUCED CHANGES IN NONPROTEINACEOUS THIOL LEVELS AND HEAVY‐METAL BINDING PEPTIDE IN TETRASELMIS TETRATHELE (PRASINOPHYCEAE)

@article{Satoh1999HEAVYMETALIC,
  title={HEAVY‐METAL INDUCED CHANGES IN NONPROTEINACEOUS THIOL LEVELS AND HEAVY‐METAL BINDING PEPTIDE IN TETRASELMIS TETRATHELE (PRASINOPHYCEAE)},
  author={Masaya Satoh and Emi Karaki and Mamiko Kakehashi and E. Okazaki and T. Gotoh and Y. Oyama},
  journal={Journal of Phycology},
  year={1999},
  volume={35}
}
The effects of mercury and cadmium on the intracellular level of nonproteinaceous thiols in a unicellular green alga Tetraselmis tetrathele (West) Butcher (Prasinophyceae) were investigated by using a fluorescent dye, 5‐chloromethylfluorescein (5CMF), as a probe for nonproteinaceous thiols. The 5CMF fluorescence was observed in cytoplasm, and the intensity of the fluorescence was decreased by exposure of the cells to HgCl2. Analysis of the fluorescent intensity of 5CMF by flow cytometry made it… Expand
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