HCLK2 is essential for the mammalian S-phase checkpoint and impacts on Chk1 stability

@article{Collis2007HCLK2IE,
  title={HCLK2 is essential for the mammalian S-phase checkpoint and impacts on Chk1 stability},
  author={Spencer J. Collis and Louise J. Barber and Allison J. Clark and Julie S. Martin and Jordan D Ward and Simon J. Boulton},
  journal={Nature Cell Biology},
  year={2007},
  volume={9},
  pages={391-401}
}
Here, we show that the human homologue of the Caenorhabditis elegans biological clock protein CLK-2 (HCLK2) associates with the S-phase checkpoint components ATR, ATRIP, claspin and Chk1. Consistent with a critical role in the S-phase checkpoint, HCLK2-depleted cells accumulate spontaneous DNA damage in S-phase, exhibit radio-resistant DNA synthesis, are impaired for damage-induced monoubiquitination of FANCD2 and fail to recruit FANCD2 and Rad51 (critical components of the Fanconi anaemia and… CONTINUE READING