HB tag modules for PCR-based gene tagging and tandem affinity purification in Saccharomyces cerevisiae.

@article{Tagwerker2006HBTM,
  title={HB tag modules for PCR-based gene tagging and tandem affinity purification in Saccharomyces cerevisiae.},
  author={Christian Tagwerker and Hongwei Zhang and Xiaorong Wang and Liza S. Z. Larsen and Richard H. Lathrop and G. Wesley Hatfield and Bernhard Auer and Lan Huang and Peter K. Kaiser},
  journal={Yeast},
  year={2006},
  volume={23 8},
  pages={623-32}
}
We have recently developed the HB tag as a useful tool for tandem-affinity purification under native as well as fully denaturing conditions. The HB tag and its derivatives consist of a hexahistidine tag and a bacterially-derived in vivo biotinylation signal peptide, which support sequential purification by Ni2+ -chelate chromatography and binding to immobilized streptavidin. To facilitate tagging of budding yeast proteins with HB tags, we have created a series of plasmids with various… CONTINUE READING
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