HAP1 positive control mutants specific for one of two binding sites.

@article{Turcotte1992HAP1PC,
  title={HAP1 positive control mutants specific for one of two binding sites.},
  author={B Turcotte and Leonard P. Guarente},
  journal={Genes & development},
  year={1992},
  volume={6 10},
  pages={2001-9}
}
The expression of the yeast CYC1 and CYC7 genes is controlled by the HAP1 activator. A GAL4-like zinc finger (residues 1-148) specifies binding to the dissimilar sites UAS1 (of CYC1) and CYC7, and an acidic domain (residues 1307-1483) is essential for activation of transcription. To analyze how HAP1 binds to UAS1 and CYC7, we performed saturation mutagenesis of the DNA-binding domain and recovered mutants with altered activity. Class 1 mutants had a reduced activity at both UAS1 and CYC7, and… CONTINUE READING