H3K36me2/3 binding and DNA binding of the DNA methyltransferase DNMT3A PWWP domain both contribute to its chromatin interaction.

@article{Dukatz2019H3K36me23BA,
  title={H3K36me2/3 binding and DNA binding of the DNA methyltransferase DNMT3A PWWP domain both contribute to its chromatin interaction.},
  author={Michael Dukatz and Katharina Holzer and Michel Choudalakis and Max Emperle and Cristiana Lungu and Pavel Bashtrykov and Albert Jeltsch},
  journal={Journal of molecular biology},
  year={2019}
}
The PWWP domain of DNMT3 DNA methyltransferases binds to histone H3 tails containing methylated K36 and this activity is important for heterochromatic targeting. Here, we show that the PWWP domain of DNMT3A binds to H3K36me2 and H3K36me3 with a slight preference for H3K36me2. PWWP domains have also been reported to bind to DNA and the close proximity of H3K36 and nucleosomal DNA suggests a combined binding to H3K36me2/3 and DNA. We show here that the DNMT3A PWWP domain binds to DNA with a weak… Expand
12 Citations
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