H2O2 release from human granulocytes during phagocytosis. I. Documentation, quantitation, and some regulating factors.

@article{Root1975H2O2RF,
  title={H2O2 release from human granulocytes during phagocytosis. I. Documentation, quantitation, and some regulating factors.},
  author={Richard K. Root and Julia Metcalf and N. Oshino and Britton Chance},
  journal={The Journal of clinical investigation},
  year={1975},
  volume={55 5},
  pages={945-55}
}
The extinction of fluorescence of scopoletin during its oxidation by horseradish peroxidase (HPO) provides a highly sensitive and specific assay for small quantities of peroxide in solution. With this assay, the release of free H2O2 into the extracellular medium by phagocytizing human granulocytes has been documented and quantitated, and some of the regulating factors have been determined. Under basal conditions granulocytes released less than 0.01 nmol/ml of H2O2 (2.5 X 10-6 polymorphonuclear… CONTINUE READING