H295R cells as a model for steroidogenic disruption: a broader perspective using simultaneous chemical analysis of 7 key steroid hormones.

@article{Nielsen2012H295RCA,
  title={H295R cells as a model for steroidogenic disruption: a broader perspective using simultaneous chemical analysis of 7 key steroid hormones.},
  author={Frederik Knud Nielsen and Cecilie Hurup Hansen and Jennifer Anna Fey and Martin Hansen and Naja Wessel Jacobsen and Bent Halling-S{\o}rensen and Erland Bj{\"o}rklund and Bjarne Styrishave},
  journal={Toxicology in vitro : an international journal published in association with BIBRA},
  year={2012},
  volume={26 2},
  pages={
          343-50
        }
}
The effects of three model endocrine disruptors, prochloraz, ketoconazole and genistein on steroidogenesis were tested in the adrenocortical H295R cell line to demonstrate that a broader mechanistic understanding can be achieved in one assay by applying chemical analysis to the H295R assay. Seven key steroid hormones (pregnenolone, progesterone, dehydroepiandrosterone, androstenedione, testosterone, estrone and 17β-estradiol) were analyzed using a novel and thoroughly validated GC-MS/MS method… CONTINUE READING

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