H-NS cooperative binding to high-affinity sites in a regulatory element results in transcriptional silencing

  title={H-NS cooperative binding to high-affinity sites in a regulatory element results in transcriptional silencing},
  author={Emeline Bouffartigues and M. Buckle and C. Badaut and A. Travers and S. Rimsky},
  journal={Nature Structural &Molecular Biology},
  • Emeline Bouffartigues, M. Buckle, +2 authors S. Rimsky
  • Published 2007
  • Biology, Medicine
  • Nature Structural &Molecular Biology
  • H-NS is a protein of the bacterial nucleoid involved in DNA compaction and transcription regulation. In vivo, H-NS selectively silences specific genes of the bacterial chromosome. However, many studies have concluded that H-NS binds sequence-independently to DNA, leaving the molecular basis for its selectivity unexplained. We show that the negative regulatory element (NRE) of the supercoiling-sensitive Escherichia coliproU gene contains two identical high-affinity binding sites for H-NS… CONTINUE READING
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