129 Citations
Proton/sodium pumping pyrophosphatases: the last of the primary ion pumps.
- BiologyCurrent opinion in structural biology
- 2014
Plant proton pumping pyrophosphatase: the potential for its pyrophosphate synthesis activity to modulate plant growth.
- BiologyPlant biology
- 2019
This work proposes a model where the PPi synthase activity of H+ -PPases maintains thePPi pool when cells adopt PPi-dependent glycolysis during high energy demands and/or low oxygen environments.
A thermostable K+‐stimulated vacuolar‐type pyrophosphatase from the hyperthermophilic bacterium Thermotoga maritima
- BiologyFEBS letters
- 2001
Pyrophosphate-Fueled Na+ and H+ Transport in Prokaryotes
- BiologyMicrobiology and Molecular Reviews
- 2013
The determination of the three-dimensional structures of H+- and Na+-pyrophosphatases has been another recent breakthrough in the studies of these cation pumps.
Functional complementation of yeast cytosolic pyrophosphatase by bacterial and plant H+-translocating pyrophosphatases
- BiologyProceedings of the National Academy of Sciences of the United States of America
- 2002
The generation of a conditional S. cerevisiae mutant (named YPC-1) whose functional IPP1 gene is under the control of a galactose-dependent promoter is described, demonstrating that hydrolysis of cytosolic PPi is essential for yeast growth and that this function is not substantially affected by the intrinsic characteristics of the PPase protein that accomplishes it.
Identification of Essential Lysines Involved in Substrate Binding of Vacuolar H+-Pyrophosphatase*
- Biology, ChemistryThe Journal of Biological Chemistry
- 2011
Proteolytic evidence demonstrated that Lys-250 is the primary target of trypsin and confirmed its crucial role in H+-PPase hydrolysis, and functional roles of these lysines were examined.
Bioinformatics Insights on Plant Vacuolar Proton Pyrophosphatase: A Proton Pump Involved in Salt Tolerance
- Biology, Environmental ScienceEssentials of Bioinformatics, Volume III
- 2019
This chapter provides an overview on bioinformatics approaches used to understand the 3D structure, motifs, function, and working model of VPPases.
Functional roles of arginine residues in mung bean vacuolar H+-pyrophosphatase.
- Biology, ChemistryBiochimica et biophysica acta
- 2007
Membrane-Bound Pyrophosphatases
- Biology
- 2015
A concise summary of the current structural and functional understanding of M-PPases is given, with an emphasis on describing the role of the metal ions in the enzymatic function of these proteins.
References
SHOWING 1-10 OF 58 REFERENCES
Vacuolar H(+)-translocating pyrophosphatases: a new category of ion translocase.
- Environmental ScienceTrends in biochemical sciences
- 1992
Isolation and Characterization of cDNAs Encoding the Vacuolar H+-Pyrophosphatase of Beta vulgaris
- BiologyPlant physiology
- 1994
Two cDNA species isolated from Beta are concluded to encode variants, possibly isoforms, of the enzyme, and the recent demonstration of the sufficiency of the substrate-binding polypeptide, alone, for all of the known catalytic functions of the V-PPase is demonstrated.
Molecular cloning of cDNA for vacuolar membrane proton-translocating inorganic pyrophosphatase in Hordeum vulgare.
- BiologyBiochemical and biophysical research communications
- 1993
A cDNA clone for the vacuolar membrane proton-translocating inorganic pyrophosphatase from barley roots was obtained by immunoscreening and the deduced amino acid sequence revealed 85.8% homology.
Reconstitution of highly purified proton‐translocating pyrophosphatase from Rhodospirillum rubrum
- Biology, ChemistryFEBS letters
- 1982
Reversibility of H+-ATPase and H+-Pyrophosphatase in Tonoplast Vesicles from Maize Coleoptiles and Seeds
- Chemistry, Biology
- 1998
Comparison of the two H + pumps showed that the H + -ATPase was more active than H -PPase in coleoptile tonoplast vesicles, whereas in seed vesicle activity was clearly dominant, which may reflect the physiological significance of these enzymes in different tissues at different stages of development and/or differentiation.
Molecular cloning and sequence of cDNA encoding the pyrophosphate-energized vacuolar membrane proton pump of Arabidopsis thaliana.
- Biology, Environmental ScienceProceedings of the National Academy of Sciences of the United States of America
- 1992
The cloning and sequence of cDNAs encoding the tonoplast H(+)-PPase of Arabidopsis thaliana are reported, and it is suggested that the H( +)- PPase has been cloned in its entirety.
Oligomeric structure of H(+)-translocating inorganic pyrophosphatase of plant vacuoles.
- BiologyBiochemical and biophysical research communications
- 1990
Heterologous expression of plant vacuolar pyrophosphatase in yeast demonstrates sufficiency of the substrate-binding subunit for proton transport.
- BiologyProceedings of the National Academy of Sciences of the United States of America
- 1994
Heterologous expression of the cDNA (AVP) encoding the substrate-binding subunit of the vacuolar H(+)-pyrophosphatase from the vascular plant Arabidopsis thaliana in the yeast Saccharomyces cerevisiae results in the production of vacu polarly localized functional enzyme active in PPi-dependent H+ translocation.
A vacuolar H+-pyrophosphatase in Acetabularia acetabulum: molecular cloning and comparison with higher plants and a bacterium
- Biology
- 1999
The primary structure of the H+-PPase from total RNA using reverse-transcription and PCR tech- was compared with the enzymes in higher plants and in the niques andignments of the primary structure to that of similar photosynthetic bacterium, R. rubrum were revealed.
Chromatographic resolution of h-translocating pyrophosphatase from h-translocating ATPase of higher plant tonoplast.
- Biology, ChemistryPlant physiology
- 1986
Membrane vesicles derived from the tonoplast of Beta vulgaris L. possess two predominant phosphohydrolase activities: a Cl(-)-stimulated, NO(3) (-)-inhibited ATPase, and a K(+)-stimulated,…