Guiding the Selection of Human Antibodies from Phage Display Repertoires to a Single Epitope of an Antigen

@article{Jespers1994GuidingTS,
  title={Guiding the Selection of Human Antibodies from Phage Display Repertoires to a Single Epitope of an Antigen},
  author={Laurent Stephane Jespers and A Roberts and Stephen M. Mahler and Gregory Paul Winter and Hennie R. Hoogenboom},
  journal={Bio/Technology},
  year={1994},
  volume={12},
  pages={899-903}
}
We have developed a strategy for guiding the selection of human antibody fragments from phage display repertoires to a single epitope of an antigen, using rodent monoclonal antibodies as a template. Thus the heavy chain of a rodent antibody (MAb32) directed against human tumor necrosis factor α (TNFα) was cloned and paired as a template chain with a repertoire of human light chains for display as Fab fragments on filamentous phage. The phage were selected by binding to the antigen. The selected… Expand
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