Guiding the Design of Synthetic DNA-Binding Molecules with Massively Parallel Sequencing

@inproceedings{Meier2012GuidingTD,
  title={Guiding the Design of
Synthetic DNA-Binding Molecules
with Massively Parallel Sequencing},
  author={Jordan L Meier and Abigail Stephanie Yu and Ian F Korf and David J Segal and Peter B. Dervan},
  booktitle={Journal of the American Chemical Society},
  year={2012}
}
Genomic applications of DNA-binding molecules require an unbiased knowledge of their high affinity sites. We report the high-throughput analysis of pyrrole-imidazole polyamide DNA-binding specificity in a 10(12)-member DNA sequence library using affinity purification coupled with massively parallel sequencing. We find that even within this broad context, the canonical pairing rules are remarkably predictive of polyamide DNA-binding specificity. However, this approach also allows identification… CONTINUE READING
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