Guide RNA-directed uridine insertion RNA editing in vitro.

  title={Guide RNA-directed uridine insertion RNA editing in vitro.},
  author={Elaine M Byrne and Gregory J Connell and Larry Simpson},
  journal={The EMBO journal},
  volume={15 23},
Guide RNAs (gRNAs) have been proposed to mediate uridine (U) addition/deletion editing of mitochondrial mRNAs in kinetoplastid protozoa. The Us are proposed to be derived either from UTP by two successive cleavage-ligations or transesterifications, or from the 3' end of the gRNA by the same mechanisms. We have demonstrated gRNA-dependent U insertions into a specific editing site of a pre-edited mRNA which was incubated in a mitochondrial extract from Leishmania tarentolae. The predominant… CONTINUE READING


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