Guanine nucleotides guanosine 5'-diphosphate 3'-diphosphate and GTP co-operatively regulate the production of an antibiotic bacilysin in Bacillus subtilis.

@article{Inaoka2003GuanineNG,
  title={Guanine nucleotides guanosine 5'-diphosphate 3'-diphosphate and GTP co-operatively regulate the production of an antibiotic bacilysin in Bacillus subtilis.},
  author={Takashi Inaoka and Kosaku Takahashi and Mayumi Ohnishi-Kameyama and Mitsuru Yoshida and Kozo Ochi},
  journal={The Journal of biological chemistry},
  year={2003},
  volume={278 4},
  pages={2169-76}
}
We found that a polycistronic operon (ywfBCDEFG) and a monocistronic gene (ywfH) are required for the biosynthesis of bacilysin in Bacillus subtilis. The disruption of these genes by plasmid integration caused loss of the ability to produce bacilysin, accompanied by a lack of bacilysin synthetase activity in the crude extract. We investigated the regulatory mechanism for bacilysin biosynthesis using the transcriptional lacZ fusion system. The transcription of these genes was found to be induced… CONTINUE READING

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