Growth phase-dependent expression of the Pseudomonas putida KT2440 transcriptional machinery analysed with a genome-wide DNA microarray.

@article{Yuste2006GrowthPE,
  title={Growth phase-dependent expression of the Pseudomonas putida KT2440 transcriptional machinery analysed with a genome-wide DNA microarray.},
  author={Luis Yuste and Ana B. Herv{\'a}s and In{\'e}s Canosa and Raquel Tobes and J. I. Jimenez and Juan Nogales and Manuel M P{\'e}rez-P{\'e}rez and Eduardo Santero and Eduardo A D{\'i}az and Juan Luis Bravo Ramos and V{\'i}ctor de Lorenzo and Fernando Rojo},
  journal={Environmental microbiology},
  year={2006},
  volume={8 1},
  pages={165-77}
}
Bacterial transcriptional networks are built on a hierarchy of regulators, on top of which lie the components of the RNA polymerase (in particular the sigma factors) and the global control elements, which play a pivotal role. We have designed a genome-wide oligonucleotide-based DNA microarray for Pseudomonas putida KT2440. In combination with real-time reverse transcription polymerase chain reaction (RT-PCR), we have used it to analyse the expression pattern of the genes encoding the RNA… CONTINUE READING
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