Growth phase‐dependent changes in the subcellular localization of pre‐B‐cell colony‐enhancing factor 1

  title={Growth phase‐dependent changes in the subcellular localization of pre‐B‐cell colony‐enhancing factor
  author={Takako Kitani and Sachiko Okuno and Hitoshi Fujisawa},
  journal={FEBS Letters},
A cDNA encoding the homolog of the human pre‐B‐cell colony‐enhancing factor (PBEF), a cytokine‐like secreted protein, was isolated from a rat cDNA library. This protein existed in both the cytoplasm and nucleus of the cells, and the amount was higher in the cytoplasm than in the nucleus of proliferating PC‐12 and Swiss 3T3 cells but higher in the nucleus than in the cytoplasm of the PC‐12 cells treated with nerve growth factor and the 3T3 cells grown to a confluent state. Thus, the so‐called… Expand
Pre‐B cell colony‐enhancing factor (PBEF)/visfatin: a novel mediator of innate immunity
A review summarizes the admittedly incomplete body of emerging knowledge about a remarkable new mediator of innate immunity about Pre‐B cell colony‐enhancing factor. Expand
Pre–B-Cell Colony–Enhancing Factor Regulates NAD+-Dependent Protein Deacetylase Activity and Promotes Vascular Smooth Muscle Cell Maturation
Findings identify PBEF as a regulator of NAD+-dependent reactions in SMCs, reactions that promote, among other potential processes, the acquisition of a mature SMC phenotype. Expand
Pre-B-cell colony-enhancing factor is a secreted cytokine-like protein from the human amniotic epithelium.
Although it had no chemotaxic effects, pre-B-cell colony-enhancing factor was antiapoptotic for both amniotic epithelial cells and fibroblasts and may protect these cells against apoptosis that is induced by chronic distension, labor, or infection. Expand
Pre-B cell colony-enhancing factor in lower vertebrates: first evidence of this cytokine being involved in antioxidant activity by reconstruction of a novel NAD salvage pathway in E. coli.
This is the first report about vertebrate pre-B cell colony-enhancing factor acting as a nicotinamide phosphoribosyltransferase to reconstruct a pyridine nucleotide cycle III pathway in E. coli and playing an important role in antioxidant stress by up-regulation of NAD biosynthesis. Expand
Pre-B cell colony enhancing factor (PBEF), a cytokine with multiple physiological functions.
The purpose of this review is to summarize the recent advances in PBEF research. Expand
Visfatin/pre-B-cell colony-enhancing factor: A protein with various suggested functions
This review summarizes the current knowledge of the various functions of PBEF/visfatin towards involvements in pathophysiology of several diseases. Expand
Nicotinamide Phosphoribosyl Transferase/Pre-B Cell Colony-Enhancing Factor/Visfatin Is Required for Lymphocyte Development and Cellular Resistance to Genotoxic Stress1
It is unequivocally demonstrated that the ability of the Nampt to regulate cell viability during genotoxic stress requires its enzymatic activity, and it may confer to cells of the immune system the ability to survive during stressful situations such as inflammation. Expand
Pre-B Cell Colony-enhancing Factor (PBEF) Promotes Neutrophil Inflammatory Function through Enzymatic and Non-enzymatic Mechanisms
Pre-B Cell Colony-Enhancing Factor (PBEF) is a cytokine-like molecule that functions as a nicotinamide phosphoribosyl transferase (Nampt) in a salvage pathway of NAD biosynthesis. PBEF hasExpand
Pre-B cell colony-enhancing factor inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis.
PBEF is identified as a novel inflammatory cytokine that plays a requisite role in the delayed neutrophil apoptosis of clinical and experimental sepsis and is also upregulated in neutrophils by IL-1beta and functions as an inhibitor of apoptosis in response to a variety of inflammatory stimuli. Expand
Crystal structure of visfatin/pre-B cell colony-enhancing factor 1/nicotinamide phosphoribosyltransferase, free and in complex with the anti-cancer agent FK-866.
The crystal structures of the complexes with either NMN or FK-866 show that the enzymatic active site of visfatin is optimized for Nicotinamide binding and that the nicotinamide-binding site is important for inhibition by FK -866, raising the intriguing possibility that the glucose metabolism and the NAD biosynthesis are linked by visFatin. Expand


Pre‐B‐cell colony‐enhancing factor, whose expression is up‐regulated in activated lymphocytes, is a nicotinamide phosphoribosyltransferase, a cytosolic enzyme involved in NAD biosynthesis
It is demonstrated that the murine homologue of the previously identified human "pre‐B‐cell colony‐enhancing factor" (PBEF) gene coding for a putative cytokine has been identified by screening a subtractive library enriched in genes expressed in activated T lymphocytes, and NAD biosynthesis may play an important role in lymphocyte activation. Expand
Cloning and characterization of the cDNA encoding a novel human pre-B-cell colony-enhancing factor.
PBEF is defined as a novel cytokine which acts on early B-lineage precursor cells and is induced by pokeweed mitogen and superinduced by cycloheximide. Expand
Genomic organization of the gene coding for human pre-B-cell colony enhancing factor and expression in human fetal membranes.
PBEF is a cytokine expressed in the normal fetal membranes and upregulated when they are infected, likely to have a central role in the mechanism of infection-induced preterm birth. Expand
Pre-B-cell colony-enhancing factor, a novel cytokine of human fetal membranes.
PBEF is a novel cytokine constitutively expressed by the fetal membranes during pregnancy and increased the expression of IL-6 and IL-8 and may be important in both normal spontaneous labor and infection-induced preterm labor. Expand
Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.
  • L. Greene, A. Tischler
  • Medicine, Biology
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1976
A single cell clonal line which responds reversibly to nerve growth factor (NGF) has been established from a transplantable rat adrenal pheochromocytoma and should be a useful model system for neurobiological and neurochemical studies. Expand
Molecular cloning of Ca2+/calmodulin-dependent protein kinase kinase beta.
Calmodulin-dependent protein kinase IV (CaM-kinase IV), which plays crucial roles in the functioning of Ca2+ in the central nervous and immune systems, is markedly activated upon phosphorylationExpand
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products. Four majorExpand
A simplification of the protein assay method of Lowry et al. which is more generally applicable.
  • G. Peterson
  • Chemistry, Medicine
  • Analytical biochemistry
  • 1977
A simple method based on a linear log-log protein standard curve is presented to permit rapid and totally objective protein analysis using small programmable calculators. Expand
DNA sequencing with chain-terminating inhibitors.
A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. Expand
Protein measurement with the Folin phenol reagent.
Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein. Expand