Growth pattern of the murein sacculus of Escherichia coli.

@article{Glauner1990GrowthPO,
  title={Growth pattern of the murein sacculus of Escherichia coli.},
  author={B Glauner and J V H{\"o}ltje},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 31},
  pages={18988-96}
}
The mechanism by which the murein sacculus of Escherichia coli is being enlarged during growth was investigated by pulse and pulse-chase labeling with [3H]diaminopimelic acid. Changes in the composition of the sacculus during aging were analyzed in detail by high performance liquid chromatography separation of the muropeptide subunits released after complete muramidase digestion. After pulses as short as 10 s, a group of novel phosphorylated muropeptides was detected. The kinetics of their… CONTINUE READING