Growth of the Sirex-parasitic nematode Deladenus siricidicola on the white rot fungus Amylostereum.

  title={Growth of the Sirex-parasitic nematode Deladenus siricidicola on the white rot fungus Amylostereum.},
  author={I. Caetano and E. Morris and A. Hajek},
  journal={Journal of invertebrate pathology},
The Kamona strain of the nematode Deladenus siricidicola has been extensively used as a biological control agent against invasive Sirex noctilio woodwasps in the Southern Hemisphere, where it sterilizes female hosts. In North America, a non-sterilizing (NS) strain of D. siricidicola, thought to have been introduced with S. noctilio, is commonly found parasitizing this invasive woodwasp. Species of Deladenus that parasitize Sirex have a parasitic form, as well as a mycophagous form. The… Expand
Efficacy of Kamona strain Deladenus siricidicola nematodes for biological control of Sirex noctilio in North America and hybridisation with invasive conspecifics
A biological control agent in North America that has successfully controlled S. noctilio in Oceania, South Africa, and South America is tested and evidence of both co-infection and hybridisation is found. Expand
Population variation in traits of Deladenus siricidicola that could influence the biocontrol of Sirex noctilio in South Africa
The 22 strains from three countries showed significant variation in the frequency of conversion into the infective form in culture, and these results emphasise the importance of considering variation in traits in D. siricidicola that could influence a biological control program. Expand
Characterisation of the dimorphic Deladenus beddingi n. sp. and its associated woodwasp and fungus
A new dimorphic species of Deladenus isolated from Sirex californicus from Washington, USA, is described as D. beddingi n. sp. Evolutionary relationships of the new species with other DeladenusExpand
Multiple introductions of Sirex noctilio (Hymenoptera: Siricidae) in northeastern North America based on microsatellite genotypes, and implications for biological control
Understanding the genetic diversity of North American S. noctilio points to new areas of inquiry, particularly regarding the ability of parasitic nematodes to sterilize woodwasps, which could provide control methods in the US and/or Canada. Expand
Minimizing the disruptive effect of Ips grandicollis (Coleoptera: Scolytinae) on biocontrol of Sirex noctilio (Hymenoptera: Siricidae)
In New South Wales, herbicide application to trap trees in January (mid-summer) best promotes S. noctilio biocontrol by minimizing colonization by I. grandicollis, but this was not as clear-cut in South Australia. Expand


Deladenus (Tylenchida: Neotylenchidae) reproduction on species and strains of the white rot fungus Amylostereum
This is the first report of the ability of D. proximus to survive and reproduce on A. areolatum, the fungal symbiont usually carried by S.noctilio, suggesting this native nematode should be evaluated for its ability to parasitize and sterilize S. noctilIO. Expand
Variability in growth of Deladenus siricidicola on strains of the white rot fungus Amylostereum areolatum
Although nematodes in all treatments produced eggs, D. siricidicola populations were unable to replace themselves when feeding on the fungal isolate SedDF, suggesting that strains of A. areolatum found in North America could affect multiple aspects of a biological control program to control S. noctilio. Expand
Parasitism of Sirex noctilio by non-sterilizing Deladenus siricidicola in northeastern North America.
The parasitic nematode Deladenus siricidicola Bedding has been extensively used for biological control of the wood wasp Sirex noctilio F, an invasive pest of pine trees in the Southern Hemisphere, and may be less effective in providing biological control compared with sterilizing D. siricicola or parasitic hymenopterans. Expand
The influence of Amylostereum areolatum diversity and competitive interactions on the fitness of the Sirex parasitic nematode Deladenus siricidicola
The results suggest that competitive interactions between A. areolatum and sapstain fungi could negatively influence the success of D. siricidicola, especially under conditions of lowered water potential. Expand
Distinguishing isolates of Deladenus siricidicola, a biological control agent of Sirex noctilio, from North America and the Southern Hemisphere using PCR–RFLP
A region from ribosomal DNA and the cytochrome oxidase subunit 1 is sequenced and a PCR–RFLP method based on a single nucleotide polymorphism flanking a microsatellite sequence is developed, useful for science-based operational biocontrol of S. noctilio. Expand
Biology of Deladenus Siricidicola (Neotylenchidae) an Entomophagous-Mycetophagous Nematode Parasitic in Siricid Woodwasps
Deladenus siricidicola has two life cycles each of which can continue indefinitely without the intervention of the other, and the time at which reproduction by the parasitic nematode commences in relation to host development may be dependant either on the species of host or the strain of nematodes involved. Expand
Fidelity Among Sirex Woodwasps and Their Fungal Symbionts
It is reported that associations between mutualistic fungi and their economically and ecologically important woodwasp hosts are not always specific as was previously assumed, and the hypothesis of strict species-specific relationships is refuted. Expand
Putative source of the invasive Sirex noctilio fungal symbiont, Amylostereum areolatum, in the eastern United States and its association with native siricid woodwasps.
Two genotypes of the fungal symbiont Amylostereum areolatum are associated with the invasive woodwasp Sirex noctilio, and nucleotide sequence data from the intergenic spacer region (IGS) of the nuclear ribosomal DNA shows genotypes found in North America are most similar to genotype found in Europe, and not to genotypes from the southern hemisphere. Expand
Lack of fidelity revealed in an insect–fungal mutualism after invasion
Investigation of the specificity of the mutualism between Sirex and Amylostereum species in Canada shows that extensive, reciprocal exchange of fungal species and strains is occurring, and partner switching may be common when shifting geographical distributions driven by ecological or anthropogenic forces bring host and mutualist pairs into sympatry. Expand
Cross-Breeding To Facilitate the Identification of Deladenus Spp., Nematode Parasites of Woodwasps
Large numbers of nematodes of seven Deladenus species from a wide range of host and geographical sources were identified by cross-breeding trials. These trials produced a spectrum of results rangingExpand