Growth of human normal erythroid progenitors in liquid culture: a comparison with colony growth in semisolid culture.

@article{Fibach1991GrowthOH,
  title={Growth of human normal erythroid progenitors in liquid culture: a comparison with colony growth in semisolid culture.},
  author={Eitan Fibach and D Manor and Adrian Treves and Eliezer A. Rachmilewitz},
  journal={International journal of cell cloning},
  year={1991},
  volume={9 1},
  pages={57-64}
}
Most studies of erythropoiesis in vitro have employed cloning methods in semisolid medium. We have recently described a two-step liquid culture procedure that supports the proliferation and differentiation of human erythroid progenitors. In the present study, we have modified the procedure to allow large-scale cultures of erythroid cells derived from normal donors. The culture is divided into two phases. In the first phase, which is erythropoietin (Epo) independent, the early erythroid… CONTINUE READING

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