Growth of exocrine acinar cells on a reconstituted basement membrane gel

@article{Oliver1987GrowthOE,
  title={Growth of exocrine acinar cells on a reconstituted basement membrane gel},
  author={Constance Oliver and Judith F. Waters and Carolyn L. Tolbert and Hynda K. Kleinman},
  journal={In Vitro Cellular & Developmental Biology},
  year={1987},
  volume={23},
  pages={465-473}
}
Methods have been developed for culturing a dividing population of morphologically differentiated rat parotid, lacrimal, and pancreatic acinar cells in vitro. Isolated acinar cells were plated onto tissue culture dishes coated with a three-dimensional, reconstituted basement membrane gel. After attachment in Ham’s nutrient mixture F12, the cells were cultured at 35°C in F12 supplemented with 10% heat inactivated rat serum, epidermal growth factor, dexamethasone, insulin, transferrin, selenium… CONTINUE READING
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Kretchmer . N . P r i m a l cultures of rat pancreatic acinar cells in serumfree medium

  • M. Brannon.P.
  • In Vitro
  • 1985

P r i m a l cultures of rat pancreatic acinar cells in serum-free medium

  • B M.Brannon.P.M.Orrison., N. Kretchmer
  • In Vitro
  • 1985
2 Excerpts

Sertoli cell differentiation, testieular cord formation, and germ cell development in vitro

  • M. A. Hadley, S. W. Byers, C. A Suares-Quian
  • J. Cell Biol
  • 1985

Cell-matrlx interaction in the embryo: cell shape, cell surface, cell skeletons, and their role in differentiation

  • E. D. Hay
  • 1984

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