Growth of dissociated rat cerebellar cells using serum-free supplemented media and varied transferrin concentrations

Abstract

Dissociated neonatal rat cerebellar cells were grown on medium supplemented with 10% horse serum (HS) and compared with those grown using a serum-free supplemented (SFS) medium, modified from Bottenstein and Sato (1979). containing insulin, transferrin, progesterone, putrescine, and selenium (after an initial 24 hr in 10% horse serum). Cells survived for… (More)
DOI: 10.1007/BF00736042

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