Growth of a pupal ectoparasitoid, Diapetimorphaintroita, on an artificial diet: stimulation of growth rate by a lipid extract from host pupae

  title={Growth of a pupal ectoparasitoid, Diapetimorphaintroita, on an artificial diet: stimulation of growth rate by a lipid extract from host pupae},
  author={S. Ferkovich and J. Shapiro and J. Carpenter},
An artificial diet used to rear the ectoparasitoid,Diapetimorpha introita, was supplemented withlipids extracted from pupae of the host, Spodoptera frugiperda (J. E. Smith). The diet alsowas sequentially supplemented with four fatty acids(arachidonic, linoleic, γ-linolenic and oleic),flax oil and Lipid Concentrate® which is used in cellculture. Pupae were homogenized and extracted withchloroform:methanol (2:1 v/v) and after drying downthe chloroform and methanol phases separately, theresidues… Expand

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