• DOI:10.1002/JOBM.200410542
• Corpus ID: 44876092

Growth of Escherichia coli at elevated temperatures

@article{Fotadar2005GrowthOE,
  title={Growth of Escherichia coli at elevated temperatures},
  author={Upinder Fotadar and Philip Zaveloff and Louis Terracio},
  journal={Journal of Basic Microbiology},
  year={2005},
  volume={45}
}

• U. Fotadar, Philip Zaveloff, L. Terracio
• Published 1 October 2005
• Chemistry
• Journal of Basic Microbiology

In our studies to develop a simple and reliable method for the generation of recombinant adenoviruses (Fotadar et al . 2003), we noted that the E. coli (BJ5183 and DH5alpha) survived a heat‐shock of 45 °C for 5 minutes in 0.1 M calcium chloride. As a result, we investigated the growth of E. coli (DH5alpha) at elevated temperatures. We hereby demonstrate that contrary to previous observations (Cooper et al . 2001 and Bronikowski et al . 2001) E. coli can grow consistently at a temperature as high as 49 °C, in spite of the fact that growth beyond 40 °C can generally be prohibitive. Hence, it is quite likely that these E. coli (DH5alpha) may have acquired mutations which permit them to grow reproducibly at 49 °C. Growth of E. coli above 49 °C (up to 53 °C) was also observed, but this was sporadic and not reproducible. This result could extend the utility of these organisms for cloning and manipulations requiring high temperature. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)