Growth kinetics of microorganisms isolated from Alaskan soil and permafrost in solid media frozen down to -35°C.

@article{Panikov2007GrowthKO,
  title={Growth kinetics of microorganisms isolated from Alaskan soil and permafrost in solid media frozen down to -35°C.},
  author={N. Panikov and M. Sizova},
  journal={FEMS Microbiology Ecology},
  year={2007},
  volume={59},
  pages={500-512}
}
We developed a procedure to culture microorganisms below freezing point on solid media (cellulose powder or plastic film) with ethanol as the sole carbon source without using artificial antifreezes. Enrichment from soil and permafrost obtained on such frozen solid media contained mainly fungi, and further purification resulted in isolation of basidiomycetous yeasts of the genera Mrakia and Leucosporidium as well as ascomycetous fungi of the genus Geomyces. Contrary to solid frozen media, the… Expand
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