Growth inhibition of subcutaneously transplanted human glioma by transfection-induced tumor necrosis factor-α and augmentation of the effect by γ-interferon

@article{Harada2005GrowthIO,
  title={Growth inhibition of subcutaneously transplanted human glioma by transfection-induced tumor necrosis factor-α and augmentation of the effect by γ-interferon},
  author={Kunyu Harada and Jun Yoshida and Masaaki Mizuno and Kenichiro Sugita and Kaoru Kurisu and Tohru Uozumi},
  journal={Journal of Neuro-Oncology},
  year={2005},
  volume={22},
  pages={221-225}
}
Using subcutaneous solid tumors produced by U251-MG human glioma cells, we studied thein vivo transfection of the cells with the tumor necrosis factor-α (TNF-α) gene delivered by means of liposomes. When the tumor had become 7 mm in diameter, liposomes with entrapped TNF-α gene were injected into the center of the subcutaneous tumor. We found that mRNA of transfection-induced TNF-α, which was expressed in the tumor tissue, was detected by reverse transcriptase-polymerase chain reaction (RT-PCR… CONTINUE READING