Growth inhibition of Staphylococcus aureus by chicken egg yolk antibodies

  title={Growth inhibition of Staphylococcus aureus by chicken egg yolk antibodies},
  author={Marco Cesar Cunegundes Guimar{\~a}es and L{\'i}via Gomes Amaral and Let{\'i}cia Batista Azevedo Rangel and Ian V. Silva and Claudia Gomes Fernandes Matta and Marcos Fernando de Rezende Matta},
  journal={Archivum Immunologiae et Therapiae Experimentalis},
The aim was to evaluate the ability of egg yolk antibody (IgY) in blocking Staphylococcus aureus growth in vitro. Specific IgY was produced by immunizing hens with formalin-killed S. aureus (ATCC 33593). Specific IgY against S. aureus was obtained from the yolks of their eggs with a carrageenan solution. IgY was identified by SDS-PAGE and Western blot and its activity against S. aureus was tested by ELISA. A growth inhibition assay and protein concentration determination were also conducted… CONTINUE READING