Growth in Tissue Culture of Analogous Mouse Mammary Carcinomas and Their Response to Radiation

  • Published 2007

Abstract

The mammary tumors used in these experiments were from two inbred strains of mice, dba and C3H, in both of which a high incidence of mammary tumors occurs. The dba strain has been inbred in the Roscoe B. Jackson Memorial Laboratory since .1918. The mammary tumor, designated dbrB, which arose in this strain was histologically diagnosed as an adenocarcinoma. It is a fast growing tumor which, implanted into hosts of the same strain, results in 100 per cent "takes" with a latent period of 4 to 6 days, and kills the host within 3 to 4 weeks. The C3H strain of mice was established by Dr. L. C. Strong and has been inbred since 1920. The tumor occurring in this strain, also histologically diagnosed as an adenocarcinoma, results in 95 to 100 per cent "takes" when implanted within hosts of the same strain. I t is a relatively slow growing tumor, with a latent period of 12 to 15 days. Figs. 1 and 2 are sections of the two tumors, both showing typical alveolar glandular structures. Numerous mitoses, which occur in both, are best shown in Fig. 1. In a previous study by one of us (3) it was found that these two analogous tumors, in hosts of their respective strains, differed widely in their radiosensitivity. Thus, implants of the dbrB tumor of the dba strain, required a dose of about 5,000 r to prevent "takes" while implants of the C3H strain required only about 2,700 r. The latent periods of the irradiated implants of the respective tumors varied significantly. For example, the longest latent period of an implant of the dbrB tumor exposed to a threshold dose of 4,500 r was 54 days, while that of the mammary tumor of the C3H strain exposed to a threshold dose of 2,600 r was 38 days. I t appears, therefore, that the ability of the tumor-implants to establish themselves in their respective hosts, and to recuperate following radiation are different. The question arises: Does the difference of these two tumors in their rate of growth and in their response to radiation depend upon specific characteristics of the tumors or does it depend upon the host? In order to obviate confusing factors in the living animal, the present study using the tissue culture method, was undertaken. This should offer information on the qualities of the tumor cells per Se. EXPERIMENTAL PROCEDURE

Cite this paper

@inproceedings{2007GrowthIT, title={Growth in Tissue Culture of Analogous Mouse Mammary Carcinomas and Their Response to Radiation}, author={}, year={2007} }