Growth impairment resulting from expression of influenza virus M2 protein in Saccharomyces cerevisiae: identification of a novel inhibitor of influenza virus.

@article{Kurtz1995GrowthIR,
  title={Growth impairment resulting from expression of influenza virus M2 protein in Saccharomyces cerevisiae: identification of a novel inhibitor of influenza virus.},
  author={Steve Kurtz and Guangxiang Luo and Karen M. Hahnenberger and Carly Brooks and O M Gecha and Kimberly A. Ingalls and Koji Numata and Mark Krystal},
  journal={Antimicrobial agents and chemotherapy},
  year={1995},
  volume={39 10},
  pages={2204-9}
}
The gene encoding M2, the ion channel-forming protein of influenza virus A, was expressed under the control of an inducible promoter in Saccharomyces cerevisiae. By using single and multicopy plasmids containing GAL promoter-M2 fusions, a correlation was observed between plasmid copy number and growth in medium inducing M2 expression. Cells expressing M2 from multicopy plasmids have reduced growth rates, suggesting that high levels of M2 are toxic to growth. The addition of amantadine, a… CONTINUE READING