Growth hormone regulates growth hormone receptor gene transcription in primary human thyroid cells

@article{Mullis2000GrowthHR,
  title={Growth hormone regulates growth hormone receptor gene transcription in primary human thyroid cells},
  author={Primus E. Mullis and Norma O'Donovan and Andr{\'e}e Ebl{\'e} and Ulrich Marti and Hans-Jakob Peter},
  journal={Molecular and Cellular Endocrinology},
  year={2000},
  volume={166},
  pages={111-119}
}
In this study the regulation of GH-receptor gene (GHR/GHBP) transcription by different concentrations of GH (0, 12.5, 25, 50, 150, 500 ng/ml) with and without variable TSH concentrations (0.5, 2, 20 mU/l) in primary human thyroid cells cultured in serum-free hormonally-defined medium was studied. The incubation time was 6 h and GHR/GHBP mRNA expression was quantitatively assessed by using PCR amplification at hourly intervals. Correlating with the GH-concentrations added a constant and… CONTINUE READING

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