Growth hormone and prolactin gene expression and release in the pituitary of rainbow trout in serum-free culture.

@article{Yada1991GrowthHA,
  title={Growth hormone and prolactin gene expression and release in the pituitary of rainbow trout in serum-free culture.},
  author={Takashi Yada and Akihisa Urano and Tetsuya Hirano},
  journal={Endocrinology},
  year={1991},
  volume={129 3},
  pages={1183-92}
}
The trout pituitary continuously released a large amount of GH for over a week in serum-free culture, but PRL secretion declined by day 2 in culture. The total amount of GH released during the 8 days exceeded the initial content. The pituitary content of PRL remained unchanged after 8 days of culture. The number of weakly stained GH-immunoreactive (ir) cells was increased by day 4 in culture. The GH mRNA levels in individual cells as revealed by in situ hybridization were maintained at around… CONTINUE READING