Growth factors that repress myoblast differentiation sustain phosphorylation of a specific site on histone H1.

A monoclonal antibody (12D11) is presented that binds an epitope on histone H1 only when it is phosphorylated. Skeletal myoblasts, which are cultured in high mitogen medium to induce proliferation and inhibit differentiation, contain histone H1 reactive with monoclonal antibody 12D11, whereas differentiated myocytes and adult skeletal muscle do not… CONTINUE READING