Growth factors in bone matrix. Isolation of multiple types by affinity chromatography on heparin-Sepharose.

  title={Growth factors in bone matrix. Isolation of multiple types by affinity chromatography on heparin-Sepharose.},
  author={Peter V. Hauschka and A E Mavrakos and Mark David Iafrati and S E Doleman and Michael Klagsbrun},
  journal={The Journal of biological chemistry},
  volume={261 27},
The mineralized matrix of osseous tissue harbors abundant mitogenic activity which is extractable by demineralizing solvents. In bovine bone powder free of blood and cartilage contamination, the volume concentration of mitogens is up to 20 times greater than in serum. Growth factor activity in bone extracts was quantitated on quiescent mouse BALB/c/3T3 fibroblasts, where [3H]thymidine incorporation for 48 h was stimulated up to 200-fold in a linear, dose-dependent manner. Six distinct bone… CONTINUE READING
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