Growth factors improve gene expression after lentiviral transduction in human adult and fetal hepatocytes.

@article{Selden2007GrowthFI,
  title={Growth factors improve gene expression after lentiviral transduction in human adult and fetal hepatocytes.},
  author={Clare Selden and Neil Mellor and Myrddin Rees and Joanna Laurson and Mike Kirwan and David Escors and Mary Collins and Humphrey Hodgson},
  journal={The journal of gene medicine},
  year={2007},
  volume={9 2},
  pages={67-76}
}
BACKGROUND Lentiviral vectors may be vectors of choice for transducing liver cells; they mediate integration in quiescent cells and offer potential for long-term expression. In adult liver, hepatocytes are generally mitotically quiescent. There has been controversy as to the necessity for lentiviral vector target cells to be in the cell cycle; currently, there is consensus that effective transduction can be achieved in quiescent hepatocytes, by using virus at high titre. However, transduction… CONTINUE READING

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