Growth characteristics of bovine herpesvirus 1 (infectious bovine rhinotracheitis) in human diploid cell strain WI-38.

@article{Michalski1976GrowthCO,
  title={Growth characteristics of bovine herpesvirus 1 (infectious bovine rhinotracheitis) in human diploid cell strain WI-38.},
  author={F. J. Michalski and Antje Dietz and Glendy Hsiung},
  journal={Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine},
  year={1976},
  volume={151 2},
  pages={407-10}
}
IBR virus was found to replicate in WI-38 cells. At a high input multiplicity the virus yield was comparable to that obtained in bovine cells, but comparable degree of CPE took longer to achieve. At a low input multiplicity of IBR virus, such as may be encountered in virus contaminated bovine serum, virus yield was only about 1% of that in bovine cells, with 50% of the cells showing CPE, followed by cell regrowth. Infectious virus was not recoverable from the regrown cells by 5 weeks after… CONTINUE READING